7240-90-6; 367-93-1; 872-50-4; 7732-18-5
Water
2-8°C
Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye. Useful in Blue-White Screening Applications.
Shelf Life: 1 Year
WARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.
0.1 lbs
2.00 x 3.50 x 1.00
Research or further manufacturing use only, not for food or drug use.
